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Feb 1, 2021 · Then, the first and the last focal plane of the Z-stack must be set, which will ideally be the focal planes slightly out-of-focus on either side of the specimen. These can be set by using the “Live” button. Further settings for Z-Stack include “slices” and “intervals”. Increasing...
Hi, welcome to my channel.This tutorial shows how to combine individual z-stack images from the
HEP2 cells using confocal microscope z-stack images with scale bar as 8 μm.
Representative Z-stack images captured by confocal microscopy (1-12) were reconstructed to a 3D
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