Nov 10, 2017 · Hi! I’m working with tissue sections stained with several fluorophores. Some of these channels are in focus at different z positions, so I’ve taken MDA images with z-stacking. In the resulting composite I know how to flip between channels and adjust their contrast if necessary. However, how...
item: File > Import > Image Sequence You’ll be presented with a folder selection dialog. Choose the
Dec 6, 2017 · Hi all, I have a multistack TIFF file with 3 channels (RGB) for each individual stack (13) - and all these are in the same exact plane. What I’m trying to do is create a singleplane …
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(Stack 1) Z position 1 and I can scroll through the 5 hours… (Stack 2) Z position 2 and i can scroll through the 5 hours.. Etc… I want to combine these stacks so that i have a scroll bar that goes through time and a scroll bar that goes...
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I am looking for the process to z-stack a sample, the software guidance gives detail on how to process it later with EDF options. Is it as simple as taking the individual images and applying them together before using the EDF step to view all in focus? ... 20 AutoQuant...
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